Gold nanorods-based FRET assay for ultrasensitive detection of DNA methylation and DNA methyltransferase activity.
نویسندگان
چکیده
A fluorescence method for the detection of DNA methylation and the assay of methyltransferase activity is proposed using gold nanorods as a fluorescence quencher on the basis of fluorescence resonance energy transfer. It is demonstrated that this method is capable of detecting methyltransferase with a detection limit of 0.25 U mL(-1), which might make this method a good candidate for monitoring DNA methylation in the future.
منابع مشابه
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عنوان ژورنال:
- The Analyst
دوره 139 18 شماره
صفحات -
تاریخ انتشار 2014